Figure 1

Schematic presentation of the experimental design of the current study. (A) Transgenic rTg4510 mice (n = 12) and their nontransgenic (ntg) littermates received daily intraperitoneal (i.p.) injections of either 25 mg/kg tubastatin or 0.9% saline. Behavior testing of all animals was performed 2 weeks prior to tissue collection. (B) Inhibition of histone deacetylase 6 increased acetylation levels of α-tubulin in vivo. Western blot analysis of brain homogenate from saline and tubastatin-treated ntg and rTg4510 animals were probed for acetylation of α-tubulin at the Lys40 residue and total α-tubulin levels. (C) Analysis of acetylated α-tubulin ratio to total α-tubulin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) by Student’s t test confirmed a significant treatment effect of tubastatin in ntg (P = 0.08 and P < 0.05, respectively) and rTg4510 animals compared with saline-treated littermates (P < 0.001 and P < 0.05, respectively). All data are presented as mean of ratio ± standard error of the mean (SEM).