Figure 3

THK523 does not bind to globose tangles in a progressive supranuclear palsy patient. Microscopy of 5-μm serial sections taken from the pons (top panels) and the striatum (bottom panels) of a representative progressive supranuclear palsy (PSP) patient. Left (Tau) images show the first of two serial sections immunostained with a tau polyclonal antibody to detect globose tangles (GB). The black arrows indicate the positioning of brown immunostained GBs in the tissue section examined. The same region of tissue was subsequently imaged for the adjacent section, which was treated with THK523 (right, THK523). The positioning of the tau lesion black arrows was transferred to the adjacent stained serial section and is indicated by the yellow arrows (THK523). The absence of fluorescence suggests that THK523 did not bind to the tau lesions of the PSP patients examined. Tissue sections were imaged using a Zeiss microscope and an AxioCam digital camera at 5× original magnification.