Figure 2

THK523 does not bind to tau lesions in corticobasal degeneration or Pick’s disease. Microscopy of 5-μm serial sections from the striatum of a corticobasal degeneration (CBD) patient (top panel) and the frontal cortex of a Pick’s disease (PiD) patient (bottom panel). The left side (Tau) shows the first of two serial sections immunostained with tau polyclonal antibodies to detect tau lesions. The arrows indicate the positioning of brown immunostained globose tangles (GT) and coiled bodies (CB; top panel) in a CBD patient and Pick’s bodies and dystrophic neurites (DN) (bottom panel). The same region of tissue was subsequently imaged for the adjacent section, which was stained with THK523 (right, THK523). The positioning of the tau lesion arrows was transferred to the adjacent stained serial section and is indicated by yellow arrows (THK523). The absence of fluorescence suggests that THK523 does not bind to CBD or PiD tau lesions. Tissue sections were imaged using a Zeiss microscope and an AxioCam digital camera at 5× (CBD) and 20× (PiD) original magnification.