J147 reduces soluble levels of Aβ. Hippocampal tissue from the aged huAPP/PS1 mice was analyzed for the effect of J147 treatment on Aβ levels. Aβ1-40 and Aβ1-42 levels were measured by ELISA in control AD animals (black bars) and AD animals fed J147 (grey bars) in the insoluble (100,000 xg pellet) (A) and RIPA soluble fractions (B and C). J147 treatment decreased insoluble Aβ1-40 and both soluble Aβ1-40 and Aβ1-42 levels. Cell lysates of hippocampal tissue from the aged huAPP/PS1 and control mice were analyzed for an effect of J147 on the APP processing pathway by immunoblotting with antibodies against BACE (D) and APP (E). The images were quantified and are represented in the bar graphs accompanying the Western blot images (D and E). BACE levels were significantly reduced upon treatment with J147 with a corresponding significant increase in APP levels. Two-tailed P- values ***P <0.001. All data shown are means ± SD, n = 10 to 11 per group. (F) Immunohistochemical analysis was done using brain coronal sections from these same mice with the antibody 6E10. Sections (30 μ thick) of similar regions from each mouse, (N = 6) were examined and plaque counts in the hippocampus were quantified. All immunohistochemical images were quantified using Image J Software. (G) The average of plaque counts for each mouse group is expressed as a number of plaques ± the SD.