Amyloid plaques in brains of mice and the effect of curcuminoid injections. (A) Time course of curcuminoid injections into Alzheimer transgenic mice. Arrowhead denotes the time point when serum-solubilized curcuminoids were switched to HP-γ-CD-solubilized curcuminoids, asterisk when i.v. injections were replaced with s.c. injections. (B) Silver stained brain section from a control non-transgenic mouse without plaques. (C) Comparable section from an Alzheimer transgenic mouse at the age of 12 months. (D) The effect of curcuminoid injections on amyloid plaque load. Left panel: Alzheimer transgenic control mice were analyzed at 12 months of age and received either no injections (n = 3), or injection of vehicle alone according to the protocols for plaque prevention (n = 3) or plaque resolution (n = 3). The differences in the relative plaque levels in each of the groups were not statistically different and the values were combined and assigned the value of 100% (n = 9). Right panel: The combined control group was compared to experimental mice that had been injected for plaque prevention (n = 6) or plaque resolution (n = 7) with a statistical difference defined at the level of P < 0.05 (*P = 0.0496).