C9orf72, amyloid beta, PHF-tau, p62, UBQLN1, and UBQLN2 immunoreactivities in Alzheimer's disease and non-Alzheimer's disease. Expression of chromosome 9 open reading frame 72 (C9orf72), amyloid beta (Aβ), PHF-tau, p62, ubiquilin (UBQLN)1, and UBQLN2 studied in the hippocampus of Alzheimer's disease (AD) and non-AD brains by double immunolabeling or single labeling of the serial sections. (a) HPA023873 (brown) and red (Aβ), AD, CA4, C9orf72-positive dystrophic neurites on amyloid plaques. (b) sc-138763 (brown) and AT8 (red), AD, C9orf72-positive PHF-tau-positive dystrophic neurites on senile plaques on the molecular layer. (c) sc-138763 (brown) and p62 (red), multiple system atrophy (MSA), C9orf72-positive p62-negative swollen dystrophic neurites in the molecular layer. (d) sc-138763 single labeling, MSA, C9orf72-positive swollen dystrophic neurites in the molecular layer. (e) UBQLN1 single labeling, MSA, area identical to (d), swollen dystrophic neurites positive for UBQLN1. (f) UBQLN2 single labeling, MSA, area identical to (d), swollen dystrophic neurites negative for UBQLN2.