Fig. 5
From: Amyloid β oligomer induces cerebral vasculopathy via pericyte-mediated endothelial dysfunction
Aβo injection inhibited PDGFRβ expression and induced an inflammatory response in PDGFRβ-cre: Ai9 mice. A Representative immunofluorescent staining images of PDGFRβ (red) and lectin (grey) in the injection region of vehicle (2%DMSO) and Aβo-injected NG2-DsRed mice. Scale bar: 20 μm B Quantification of the total length of lectin+ area. C-D Quantification of PDGFRβ.+ coverage length (left panel) and area (right panel) in the injection region of both groups. E–F Western blotting analysis of PDGFRβ protein expression level in brain tissues after 2%DMSO and Aβo injection. G QPCR analysis of PDGFRβ mRNA expression level in brain tissues after vehicle and Aβo injection. H QPCR analysis of mRNA levels of some classic inflammatory factors (IL6, IL10, TNFα, CCL2, CXCL10) in brain tissues. All the data were presented as mean ± SEM and analyzed using an unpaired t-test. n = 6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001