Fig. 3

Aβ oligomer stereotactic injection induced BBB leakage and local cortex hypoperfusion in 2mo WT mice. A Schematic diagram of the experiment design. B Representative in vivo two-photon images of the cortex of mice in control (n = 4), vehicle (2%DMSO) (n = 6), and Aβo-injected (n = 7) groups. 2–3 stacks were acquired to obtain the mean for each animal. Scale bar: 100 μm C. Variation curve of extravasated FITC-dextran dye intensity in these three groups after injection. D Quantitative analysis of FITC-dextran extravasation 30 min after injection. E–F Quantification of cortical vascular length and area from the three experimental groups. G Representative laser speckle images of DMSO (n = 8) and Aβo (n = 11) groups. H Quantification of D-value of cerebral perfusion between injection region (ROI 1) and contralateral cortex (ROI 2) in DMSO and Aβo groups. D-value was defined as the ROI 1 quantitative perfusion value minus ROI 2. All the data were presented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001