Fig. 2
From: Amyloid β oligomer induces cerebral vasculopathy via pericyte-mediated endothelial dysfunction
Vascular PDGFRβ deficiency was found in the brains of 8-10mo 5xFAD mice. A Representative immunofluorescent staining images of PDGFRβ (red), Aβ (green) and lectin (grey) co-labeling in the hippocampus (upper panel) and cortex (low panel) of 8-10mo wild type (n = 6) and 5xFAD (n = 8) mice. Scale bar: 50 μm B. Quantification of Aβ-positive area in both groups. C Quantification of PDGFRβ-positive coverage area. D Pearson’s coefficient (r) correlation analysis between the percentage of PDGFRβ+ area and Aβ.+ area in the cortex of AD mice. E Quantitative analysis of PDGFRβ mRNA levels. n = 4 for each group. F-G Western blotting analysis of PDGFRβ protein expression level in the hippocampus (upper panel) and cortex (low panel) of wild type (n = 4) and 5xFAD mice (n = 6). Data are shown as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001