Fig. 1
From: Amyloid β oligomer induces cerebral vasculopathy via pericyte-mediated endothelial dysfunction
Vasculopathy was observed in the brains of 8-10mo 5xFAD mice. A Representative two-photon images showed cerebral vasculature and BBB breakdown in the somatosensory cortex of 8-10mo 5xFAD mice. 70kD Rhodamine-dextran was shown in red and 4kD FITC-dextran dye was in green. Scale bar: 100 μm B. Variation curve of the total extravascular 4kD FITC-dextran signal intensity after retro-orbital injection. C Volumetric quantification of FITC-dextran extravasation 30 min after injection. D-E Quantitative analysis of total vessel coverage area and length in the cortex of both groups. F Representative laser speckle images demonstrated the situation of cerebral perfusion. G Quantification of cerebral perfusion in ROI. All the data were presented as mean ± SEM and analyzed using unpaired t test. n = 6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001