Fig. 4

Histological staining of NFT pathological features in the brain of T2DM monkeys. A-B Images of DAB staining in the FC, TC, TETC, and HIP of representative symptomatic T2DM group (A) and NC group (B) showing p-tau immunoreactivity. C-D Quantification of p-tau in the brain of the T2DM group and NC group. E Levels of p-tau 231 in the cortex of monkeys were measured using Western blot analysis. β-Actin was used as a loading control. Data are represented as mean ± SD. Statistical differences are evaluated with a two-tailed unpaired Student’s t-test (n = 6/T2DM group; n = 3/NC group) (p > 0.05 = ns; p ≤ 0.05 = *; p ≤ 0.01 = **; p ≤ 0.001 = ***). The arrowheads indicate neuropil threads and empty arrowheads indicate droplet-like inclusions. Scale bars: 50 μm