Table 1 Animal information summary
Animal No | Sex | Age (year) | Virus injection | The experiments that the animal was used for |
|---|---|---|---|---|
07379 | M | 16 | AAV expressing hTau + AAV expressing GFP | Immunostaining (GFP), Immunostaining (hTau/pTau/3R Tau/4R Tau), CSF collection & analysis (AD biomarkers), PET imaging (Tau/FDG), MRI (hippocampal atrophy), Thioflavin S staining, Behavioral tests (learning, memory) |
08019 | M | 15 | AAV expressing hTau + AAV expressing GFP | Immunostaining (GFP), Immunostaining (hTau/pTau/3R Tau/4R Tau), CSF collection & analysis (AD biomarkers), PET imaging (Tau/FDG), MRI (hippocampal atrophy), Thioflavin S staining, Behavioral tests (learning, memory) |
08061 | M | 15 | AAV expressing hTau | Immunostaining (hTau/pTau/3R Tau/4R Tau), PET imaging (Tau/FDG), Immunostaining (NeuN/Tuj1/MAP2/GFAP/Iba1/CD68/Laminin/PECAM1/AQP4), Nissl staining, Thioflavin S staining |
08383 | M | 15 | AAV expressing hTau + AAV expressing GFP | Immunostaining (hTau/pTau/3R Tau/4R Tau), CSF collection & analysis (AD biomarkers), PET imaging (Tau/FDG), MRI (hippocampal atrophy), Thioflavin S staining, Behavioral tests (learning, memory) |
09016 | F | 14 | AAV expressing hTau + AAV expressing GFP | Immunostaining (GFP), Immunostaining (hTau/pTau/3R Tau/4R Tau), CSF collection & analysis (AD biomarkers), PET imaging (Tau/FDG), MRI (hippocampal atrophy), Thioflavin S staining, Behavioral tests (learning, memory) |
09078 | F | 14 | AAV expressing hTau + AAV expressing GFP | Immunostaining (GFP), Immunostaining (hTau/pTau/3R Tau/4R Tau), CSF collection & analysis (AD biomarkers), PET imaging (Tau/FDG), MRI (hippocampal atrophy), Thioflavin S staining, Behavioral tests (learning, memory) |
14189 | M | 9 | AAV expressing hTau + AAV expressing GFP | Immunostaining (GFP), Immunostaining (hTau/pTau/3R Tau/4R Tau), PET imaging (Tau/FDG), Immunostaining (NeuN/Tuj1/MAP2/GFAP/Iba1/CD68/Laminin/PECAM1/ AQP4), Nissl staining |
16167 | M | 7 | AAV expressing hTau + AAV expressing GFP | Immunostaining (GFP), Immunostaining (hTau/pTau/3R Tau/4R Tau), PET imaging (Tau/FDG), Immunostaining (NeuN/Tuj1/MAP2/GFAP/Iba1/CD68/Laminin/PECAM1/ AQP4) |
16195 | M | 7 | Control AAV + AAV expressing GFP | Immunostaining (GFP), Immunostaining (hTau/pTau/3R Tau/4R Tau), Immunostaining (NeuN/Tuj1/MAP2/GFAP/Iba1/CD68/Laminin/PECAM1/AQP4), Nissl staining |
16197 | M | 7 | Control AAV + AAV expressing GFP | Immunostaining (GFP), Immunostaining (hTau/pTau/3R Tau/4R Tau), Immunostaining (NeuN/Tuj1/MAP2/GFAP/Iba1/CD68/Laminin/PECAM1/AQP4), Nissl staining |
134021 | M | 10 | AAV expressing hTau | Immunostaining (hTau/pTau/3R Tau/4R Tau), Immunostaining (NeuN/Tuj1/MAP2/ GFAP/Iba1/CD68/Laminin/PECAM1/AQP4), Nissl staining |
134023 | M | 10 | AAV expressing hTau | Immunostaining (hTau/pTau/3R Tau/4R Tau), Immunostaining (NeuN/Tuj1/MAP2/ GFAP/Iba1/CD68/Laminin/PECAM1/AQP4), Nissl staining |
134041 | M | 10 | AAV expressing hTau | Immunostaining (hTau/pTau/3R Tau/4R Tau), Immunostaining (NeuN/Tuj1/MAP2/ GFAP/Iba1/CD68/Laminin/PECAM1/AQP4), Nissl staining |
08111401 | M | 15 | Control AAV | Immunostaining (hTau/pTau/3R Tau/4R Tau), Thioflavin S staining, Behavioral tests (learning, memory) |
09185 | M | 14 | Control AAV | Immunostaining (hTau/pTau/3R Tau/4R Tau), Thioflavin S staining, Behavioral tests (learning, memory) |
13041923 | M | 10 | Control AAV | Immunostaining (hTau/pTau/3R Tau/4R Tau), Immunostaining (NeuN/Tuj1/MAP2/ GFAP/Iba1/CD68/Laminin/PECAM1/AQP4), Nissl staining, Thioflavin S staining, Behavioral tests (learning, memory) |
14102103 | M | 9 | Control AAV | Immunostaining (hTau/pTau/3R Tau/4R Tau), Immunostaining (NeuN/Tuj1/MAP2/ GFAP/Iba1/CD68/Laminin/PECAM1/AQP4), Nissl staining, Thioflavin S staining, Behavioral tests (learning, memory) |
16030703 | M | 7 | Control AAV | Immunostaining (hTau/pTau/3R Tau/4R Tau), Immunostaining (NeuN/Tuj1/MAP2/ GFAP/Iba1/CD68/Laminin/PECAM1/AQP4), Nissl staining, Thioflavin S staining, Behavioral tests (learning, memory) |