Fig. 6

CASP11 promotes IL-1β release in response to Aβ by facilitating cleavage of GSDMD and CASP1. A IL-1β release from LPS-primed macrophages from wild-type (WT), casp4^−/−, gsdmd^−/−, and casp1^−/− mice treated for 3 h with 10-µM Profect-conjugated fibrillar-Aβ or Profect alone (NT + Profect) (N = 9). B Representative immunoblots for cleaved GSDMD (30 kb), cleaved CASP1 (20 kb), and GAPDH (37 kb) from macrophages lysates treated with Profect-conjugated fibrillar-Aβ (fAβ) or Profect alone (NT) as in A. C Densitometry analysis of immunoblots for cleaved GSDMD and cleaved CASP1 relative to GAPDH above background levels for macrophages treated as in A and B (N = 8 for WT and casp4^−/−, N = 3 for gsdmd^−/−, and casp1.^−/−). Statistical analysis for A and C completed by two-way ANOVA Tukey’s multiple comparisons test. For simplicity, graphs do not display P-values for all comparisons. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001