Fig. 5

Profect-conjugated-fibrillar-Aβ (1–42) stimulates release of IL-1β and cleavage of Gasdermin-D and Caspase-1. A IL-1β release from LPS-primed mouse macrophages treated for 3 h with 10-µM fibrillar-Aβ (fAβ), 10-µM scramble-Aβ (sAβ), 10-µM monomer-Aβ (mAβ), and 250 ng/mL flagellin control or not treated (NT) with and without conjugation to cytosolic delivery reagent Profect (N = 10). B Representative immunoblots for cleaved GSDMD (30 kb), cleaved CASP1 (20 kb), and GAPDH (37 kb) from macrophages lysates treated as in A. C–F Densitometry analysis of immunoblots for cleaved GSDMD and cleaved CASP1 relative to GAPDH above background levels from LPS-primed mouse macrophages treated for 3 h with C 10-µM fibrillar-Aβ with and without Profect, D 10-µM scramble-Aβ with and without Profect, E 10-µM monomer-Aβ with and without Profect, and F 250 ng/mL flagellin with and without Profect (N = 6). Statistical analysis for A and C–F completed by two-way ANOVA Tukey’s multiple comparisons test with matching. For simplicity, graphs do not display P-values for all comparisons. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001