Fig. 3

CASP11 deficiency leads to decreased Aβ deposition and reduced microglial release of IL-1β in 5xFAD mice. Immunoblots for Aβ and GAPDH loading control in hippocampal lysate from 7-month-old littermate WT, 5xFAD, Casp4^−/−, and 5xFAD/Casp4^−/− for A male and B female mice. Densitometry analysis of immunoblots for Aβ relative to GAPDH above background is displayed. Statistical analysis completed by Student’s t-test. *P ≤ 0.05. C Immunohistochemistry of Aβ (green) and DAPI (blue) in the hippocampus of 5xFAD and 5xFAD/Casp4^−/− mice. D IL-1β in cell culture supernatants from 5 and 5xFAD/Casp4^−/− microglia from 8- to 10-month-old male and female mice. Statistical analysis completed mixed-effect analysis with Sidak multiple comparison test (N = 6). *P < 0.05, **P < 0.005. E Percentage of microglia (CD45^int CD11b^high) among the live cell population and percentage of microglia expressing high levels of CD86 from whole brain homogenates 7-month-old littermate WT, 5xFAD, and 5xFAD/Casp4.^−/− mice. Statistical analysis for completed by two-way ANOVA Tukey’s multiple comparisons test. *P ≤ 0.05