Fig. 2

CASP11 exhibits increased expression in the 5xFAD microglia. A Relative copy number (RCN) of Casp4 in homogenized mouse brains from 7-month-old 5xFAD and age- and sex-matched wild-type (WT) littermate controls and 5xFAD male mice compared to female mice determined by RT-PCR. RCNs are relative to housekeeping gene GAPDH and multiplied by a factor of 100. Statistical analysis was completed by Students t-test. B Immunoblots for CASP11 and β-actin loading control in microglia (CD11b +) and non-microglia (CD11b −) fractions from 5-month-old WT and 5xFAD mice. C Densitometry of CASP11 relative to GAPDH above background in microglia (CD11b +) for immunoblot shown in B (N = 3). Statistical analysis completed by Student’s t-test. *P ≤ 0.05