Fig. 6
Blockade of A2AR Reversed the Alterations in Dendritic Morphology of abGCs in APP/PS1 Mice. A Representative images of 1.5-month post-injection (mpi) GFP + abGCs from different groups of mice. Upper: low magnification image showing the location of GFP + abGCs, scale bar = 100 μm. White dotted lines were added to mark the boundary line between the granule cell layer (GCL), molecular layer (ML), and hilus. Middle: representative maximal projection for dendritic morphological analysis, scale bar = 20 μm. Lower: high-power magnification image showing examples of dendritic spines, scale bar = 5 μm. B Quantification of dendritic arborization using Sholl analysis showed that abGCs of APP/PS1 mice displayed a significant decrease in dendritic branching compared to WT mice, which was reversed by SCH58261 treatment, n = 7 neurons per group, with each neuron from an individual animal. C Histograms showing a decrease in total dendritic length for abGCs of APP/PS1 mice compared to WT mice. However, treatment with SCH58261 partially reversed the decrease in the total dendritic length for abGCs of APP/PS1 mice. D Histograms showing a significant reduction in dendritic spine density in abGCs of APP/PS1 mice compared to WT mice, which was reversed by SCH58261 treatment, n = 9 neurons per group, with each neuron from an individual animal. Data are presented as the mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 by repeated-measures ANOVA or one-way ANOVA followed by Sidak's post-hoc test