Fig. 7
From: PACAP–Sirtuin3 alleviates cognitive impairment through autophagy in Alzheimer’s disease
Three autophagy pathways were affected by PACAP treatment in 3xTg mice and HT22 cells. Western blots and corresponding graphs show protein levels of (A) p-PI3K:PI3K ratio (n = 5), (B) p-AKT:AKT ratio (n = 5), (C) p-mTOR:mTOR ratio (n = 5), (D) p-AMPK:AMPK ratio (n = 5), and (E) Sirt3 (n = 5) in mouse hippocampus. Western blots and corresponding graphs show protein levels of (F) p-PI3K:PI3K ratio (n = 4), (G) p-AKT:AKT ratio (n = 6), (H) p-mTOR:mTOR ratio (n = 5), (I) p-AMPK:AMPK ratio (n = 6), and (J) Sirt3 (n = 5) in HT22 cells, n = 5. *p < 0.05, **p < 0.01, ***p < 0.001