Fig. 6

Pathological protein accumulation was alleviated by PACAP treatment in vivo and in vitro. Western blots and corresponding graphs show protein levels of (A) PACAP (n = 4), (B) Aβ (n = 3), and (C) total Tau (n = 5). Immunohistochemical staining of Aβ in the hippocampus of WT (D and Di), 3xTg (E and Eii), and 3xTg + PACAP mice (F and Fiii). Scale bar, 2 μm. G Quantitative analysis of Aβ density (n = 3). Immunohistochemical staining of pTau in the hippocampus of WT (H and Hi), 3xTg (I and Iii), and 3xTg + PACAP mice (J and Jiii). Scale bar, 2 μm. K Quantitative analysis of pTau fluorescence density (n = 3). Western blots and corresponding graphs show protein levels of (L) Aβ (n = 4) and (M) total Tau in HT22 cells, n = 5. *p < 0.05, **p < 0.01, ***p < 0.001