Fig. 3
From: Progressive mechanical and structural changes in anterior cerebral arteries with Alzheimer’s disease
Representative histological and multiphoton images at the circumferential cross section of ACAs showing progressive cellular and extracellular structural changes with AD progression. a, e, i 72-year-old male (control), b, f, j 72-year-old male (Braak II to III, early AD), c, g, k 69-year-old male (Braak IV, intermediate AD), and d, h, l 79-year-old male (advanced AD). a-d Histological images with Movat’s stain (nuclei and elastin black, GAGs blue, and collagen yellow). e-l Maximum intensity projection multiphoton images with single elastin channel (e–h) and combined collagen and elastin channels (i-l) with collagen fibers in red and elastic fibers in green. The internal elastic lamella (IEL) of the ACAs faces upward in all images