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Fig. 8 | Alzheimer's Research & Therapy

Fig. 8

From: Feasibility and therapeutical potential of local intracerebral encapsulated cell biodelivery of BDNF to AppNL−G−F knock-in Alzheimer mice

Fig. 8

Flow cytometry and immunofluorescence analysis of the neuroinflammatory response to ECB implants at 4 months. A ECB-adhering cells at post-explantation were collected, pooled, and surface stained for various immune cell markers and analyzed by flow cytometry. Pan-leukocyte marker CD45 was used to select immune cells and was sub-gated to identify specific cell types including T-, natural killer (NK)-, B-, dendritic (DC)-, and microglia-macrophage (CD11b) cells, respectively. B Representative immunofluorescence images of BDNF levels, microglia (Iba1), astrocytes (GFAP), leukocytes (CD45), and Aβ plaques staining. Scale bar 300 μm. C Specific staining were analyzed in the proximity and in the distal area surrounding the ECB devices and presented as bar-plots (n = 4–5). Data are represented as mean ± S.E.M. Data were analyzed by unpaired Student’s t-test

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Alzheimer's Research & Therapy

ISSN: 1758-9193