Fig. 10

Treatment with TOMA did not affect insoluble tau detected by western immunoblotting analysis. Anterior cortex was homogenized in RIPA buffer and the detergent insoluble fraction prepared by centrifugation of the homogenate. The pellet was solubilized with formic acid and neutralized with NaOH prior to electrophoresis. a–d Representative immunoblots for antibodies pSer396, H150, pSer 199/202, and pSer262 respectively. Data were quantified for three molecular weight size ranges for each antibody and normalized to total protein visualized with the Revert reagent (e–h). Data expressed as mean ± SEM. TOMA tau oligomer monoclonal antibody. MW Kda molecular weight in kilodaltons. HMW high molecular weight (100-160 kilodaltons)