Fig. 2
From: Tau passive immunization inhibits not only tau but also Aβ pathology
Immunization with tau antibodies 43D and 77E9 rescues cognitive impairment without any side effects in 3×Tg-AD mice. a The body weights of the mice were measured once per week. b–f The Morris water maze test was carried out after the sixth immunization (see Fig. 1). b The escape latency (in seconds) to reach the hidden platform during acquisition phase for 5 days (two-way ANOVA, WT-IgG vs. 3×Tg-IgG, F = 8.47, dfd = 90, p < 0.01; 3×Tg-IgG vs. 3×Tg-43D, F = 12.95, dfd = 75, p < 0.001; 3×Tg-IgG vs. 3×Tg-77E9, F = 0.23, dfd = 70, p = 0.63). c Percentage of time in the quadrant during the probe trial (two-way ANOVA, F = 241.59, dfd = 200, p < 0.0001; Bonferroni posttests for target quadrant, WT-IgG vs. 3×Tg-IgG, p < 0.01; 3×Tg-IgG vs. 3×Tg-43D, p < 0.001; 3×Tg-IgG vs. 3×Tg-77E9, p < 0.01). d Number of target crossings in the probe trial (one-way ANOVA, F = 3.666, p = 0.0067; Bonferroni multiple comparisons test, WT-IgG vs. 3×Tg-IgG, p < 0.05; 3×Tg-IgG vs. 3×Tg-43D, p < 0.01; 3×Tg-IgG vs. 3×Tg-77E9, p < 0.01). e Latency to first entrance into target zone (one-way ANOVA, F = 2.717, p = 0.0301; Bonferroni multiple comparisons test, WT-IgG vs. 3×Tg-IgG, p < 0.05; 3×Tg-IgG vs. 3×Tg-43D, p < 0.05). f The average swim speed during the probe trial (one-way ANOVA, F = 1.003, p = 0.4257). g and h, One-trial novel object recognition test (see Fig. 1). g The percentage of time spent exploring two identical objects during sample phase (two-way ANOVA, F = 18.93, dfd = 122, p < 0.0001). h Discrimination index (time spent exploring novel object/time spent exploring novel and familiar objects) × 100% in test phase (one-way ANOVA, F = 4.517, p = 0.0014; Bonferroni multiple comparisons test, WT-IgG vs. 3×Tg-IgG, p < 0.01; 3×Tg-IgG vs. 3×Tg-43D, p < 0.001; 3×Tg-IgG vs. 3×Tg-77E9, p < 0.01). n = 12 for WT mice treated with IgG or 43D + 77E9, 3×Tg-AD mice treated with 43D, 77E9, or 43D + 77E9; and n = 14 for 3×Tg-AD mice treated with IgG. Data are reported as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 by ANOVA followed by a Bonferroni post hoc test. AD Alzheimer’s disease, ANOVA Analysis of variance, IgG Immunoglobulin G, 3×Tg Triple-transgenic, WT Wild type