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Table 3 Summary of key studies on electrophysiological profiles of human induced neuronal cells

From: Connectivity and circuitry in a dish versus in a brain

 

Ambasudhan and colleagues, 2011 [97]

Caiazzo and colleagues, 2011 [119]

Pang and colleagues, 2011 [99]

Pfisterer and colleagues, 2011a [100]

Pfisterer and colleagues, 2011b [100]

Son and colleagues, 2011 [149]

 

Yoo and colleagues, 2011 [101]

Koppensteiner and colleagues, 2014 [108]

Method

hiN

hiN

miN

hiN

hiN

hiN

hiN

hiN

hiN

hiN

hiN

hiN

hiN

miN

hiN

hiN

hiN

hiN

Age of fibroblast donor

Neonatal

Adult

Embryonic

>40 years

Fetal

Fetal

Neonatal

11 years

Embryonic

Fetal

Fetal

Embryonic

Adult

Embryonic

Embryonic

Neonatal

30 years

Adult

Number of cell lines

2

2

1

4

3

3

3

1

1

1

1

1

3

1

1

1

1

1

Viral factors

Brn2, Myt1l, miR-124

Ascl1, Nurr1, Lmx1a

BAM + NeuroD1

BAM + Lmx1a + FoxA2

BAM

BAM

BAM + four factors

BAM + five factors

miR-9/9*, miR-124, DAM

BAM + Zic1

Conversion efficiency (%)

4 to 8

1.5 to 11

2

3 to 5

2 to 4

2 to 4

2 to 4

NQ

5

10

13

16

1 to 4

NQ

NQ

NQ

NQ

NQ

DIV

25 to 30

30

16

18

14 to 25

34 to 35

28 to 35

21

30 to 32

30 to 32

30 to 32

30 to 32

23 to 34

NQ

NQ

35 to 56

35 to 56

21 to 28

AP firing (% of cells)

81

12

NQ

NQ

21

100

79

NQ

86

83

79

91

47

90

NQ

85

60

63

Single (%)

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

62

Repetitive (%)

20

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

NQ

77

56

7

Spontaneous AP (%)

15

NQ

81

NQ

2

0

3.0

NQ

29

0

NQ

NQ

NQ

NQ

NQ

10

NQ

0

n

29

25

16

8

42

5

29

NQ

7

6

14

12

60

10

NQ

27

15

27

Rin (MΩ)

405.0

1,067.0

1,100.0

NQ

1,770.0

1,110.0

1,500.0

x

157.0

152.0

966.0

843.0

x

NQ

NQ

2,600.0

2,800.0

1,060.0

Cm (pF)

30.5

35.9

10.6

NQ

16.0

25.1

23.0

x

NQ

NQ

NQ

NQ

x

NQ

NQ

16.3

22.9

33.3

Ï„ (milliseconds)

NQ

NQ

NQ

NQ

NQ

NQ

NQ

x

NQ

NQ

NQ

NQ

x

NQ

NQ

NQ

NQ

35.4

RMP (mV)

−45.0

x

−41.8

NQ

−52.1

−59.5

−41.0

x

−45.3

−41.6

−62.4

−58.6

x

−49.5

NQ

−42.0

−31.4

−42.1

n

21

21

16

8

52

21

29

x

7

6

14

12

x

6

NQ

27

15

27

Spontaneous activity (%)

25

43

x

x

0

19

33

x

x

x

x

x

x

x

x

71

84

0

Evoked potential (%)

x

x

x

x

0

14

0

x

x

x

x

x

x

x

x

45

47

0

n

8

7

x

x

20

21

6

x

x

x

x

x

x

x

x

12 to 14

10 to 12

9 to 57

Co-culture

x

x

x

x

Mouse cortical neurons

x

x

x

x

x

x

x

x

Mouse glial cells

x

   
  1. Note the more immature electrophysiological properties of neurons converted from adult somatic cells compared with those converted from fetal or neonatal tissues. AP, action potential; BAM, Brn2, Ascl1, Myt1l; DAM, NeuroD2, Ascl1, Myt1l; Cm, membrane capacitance; DIV, days in vitro; five factors, Lhx3, Hb9, Isl1, Ngn2, NeuroD1; four factors, Lhx3, Hb9, Isl1, Ngn2; hiN, human induced neuronal; miN, mouse induced neuronal; NQ, not quantified; Rin, input resistance; RMP, resting membrane potential; Ï„, membrane time constant; x, not measured, used or stated
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Alzheimer's Research & Therapy

ISSN: 1758-9193