Characterization of the chromatin accessibility in an Alzheimer’s disease (AD) mouse model

Background The pathological hallmarks of Alzheimer’s disease (AD) involve alterations in the expression of numerous genes associated with transcriptional levels, which are determined by chromatin accessibility. Here, the landscape of chromatin accessibility was studied to understand the outline of the transcription and expression of AD-associated metabolism genes in an AD mouse model. Methods The assay for transposase-accessible chromatin by sequencing (ATAC-seq) was used to investigate the AD-associated chromatin reshaping in the APPswe/PS1dE9 (APP/PS1) mouse model. ATAC-seq data in the hippocampus of 8-month-old APP/PS1 mice were generated, and the relationship between chromatin accessibility and gene expression was analyzed in combination with RNA sequencing. Gene ontology (GO) analysis was applied to elucidate biological processes and signaling pathways altered in APP/PS1 mice. Critical transcription factors were identified; alterations in chromatin accessibility were further confirmed using chromatin immunoprecipitation assays. Results We identified 1690 increased AD-associated chromatin-accessible regions in the hippocampal tissues of APP/PS1 mice. These regions were enriched in genes related to diverse signaling pathways, including the PI3K-Akt, Hippo, TGF-β, and Jak-Stat signaling pathways, which play essential roles in regulating cell proliferation, apoptosis, and inflammatory responses. A total of 1003 decreased chromatin-accessible regions were considered to be related with declined AD-associated biological processes including cellular response to hyperoxia and insulin stimulus, synaptic transmission, and positive regulation of autophagy. In the APP/PS1 hippocampus, 1090 genes were found to be upregulated and 1081 downregulated. Interestingly, enhanced ATAC-seq signal was found in approximately 740 genes, with 43 exhibiting upregulated mRNA levels. Several genes involved in AD development were found to have a significantly increased expression in APP/PS1 mice compared to controls, including Sele, Clec7a, Cst7, and Ccr6. The signatures of numerous transcription factors, including Olig2, NeuroD1, TCF4, and NeuroG2, were found enriched in the AD-associated accessible chromatin regions. The transcription-activating marks of H3K4me3 and H3K27ac were also found increased in the promoters of these genes. These results indicate that the mechanism for the upregulation of genes could be attributed to the enrichment of open chromatin regions with transcription factors motifs and the histone marks H3K4me3 and H3K27ac. Conclusion Our study reveals that alterations in chromatin accessibility may be an initial mechanism in AD pathogenesis. Supplementary information Supplementary information accompanies this paper at 10.1186/s13195-020-00598-2.


Background
Alzheimer's disease (AD), an age-associated chronic progressive neurodegenerative disorder, is characterized by progressive loss of memory, cognitive impairment, and behavioral changes. It is the most common form of dementia. A global systematic analysis of AD from 1990 to 2016 that included 195 countries showed that the global number of individuals who lived with dementia was 43.8 million in 2016, showing a 117% increase compared to 1990 [1]. In 2016, dementia was the fifth largest cause of death globally, accounting for 4.4% of total mortality. Notably, 8.6% of deaths were observed in individuals aged more than 70 years, making dementia the second largest cause of death in this age group [2].
The pathological hallmarks of AD are characterized by β-amyloid (Aβ) deposition, neurofibrillary tangles induced by phosphorylation of tau protein, upregulation of inflammation, and neuronal apoptosis. All these processes involve alterations in the expression and regulation of numerous genes. The Apolipoprotein E (APOE) gene is a well-known genetic risk factor identified for AD. The association of the APOE ε4 allele with AD risk has been repeatedly demonstrated by different studies, whereas the ε2 allele has been found to be related to a protective effect [3,4]. Rare mutations in APP, PSEN1, and PSEN2 are discovered to be associated with autosomal dominant familial AD, which is found in about 70% of familial AD patients [5][6][7]. With the development of next-generation sequencing (NGS) over the past few years, multiple AD genes have been identified. Rare variants in TREM2 are considered a major genetic risk factor for AD [8][9][10]. In addition, both SORL1 and ABCA7 are demonstrated to carry numerous loss-offunction variants leading to strong increases in AD risk [11][12][13][14]. Moreover, a study from the Alzheimer's disease sequencing project, which encompassed more than 5000 cases and controls, reported two new candidate genes, IGHG3 and ZNF655 [15]. Expression levels of AD genes are important in AD etiology; however, information on how they are specifically regulated is still limited. Thus, exploring the regulatory elements of AD genes and their corresponding transcription factors (TFs) is critically important for elucidation of the disease process.
In recent years, it has been recognized that chromatin is a dynamic central regulator of transcription. The chromatin structure defines the scenario where interactions between TFs and their cognate regulatory regions take place. To successfully interact with cis-regulatory elements, such as promoters, enhancers, insulators, and non-coding RNAs (ncRNAs), TFs must induce chromatin remodeling of nucleosomal structures, which results in different levels of chromatin accessibility [16,17]. Therefore, open chromatin regions allow transcription machinery components to access to cis-regulatory elements and activate gene transcription, while closed chromatin regions impair the accessibility of promoters and enhancers to transcription factors and other regulators of transcription inducing gene silencing.
Recently, the results of several studies have demonstrated substantial changes in chromatin accessibility in human brains [18][19][20]. McClymont et al. reported the alteration of open chromatin in mouse dopamine neurons and found Parkinson-associated SNCA enhancer variants, indicating the utility of chromatin accessibility in studying the regulation of gene expression in neurodegenerative diseases [21]. However, the role of chromatin remodeling in AD processes has not been investigated yet.
In this work, we used the Assay for Transposase-Accessible Chromatin by sequencing (ATAC-seq), a sensitive tool for integrative epigenomic analysis, combined with RNA sequencing (RNA-seq) to investigate the pattern of genome-wide chromatin accessibility in AD in an APP/PS1 mouse model, which resembles the familial AD in humans, and characterize the chromatin accessibility landscape in APP/PS1 mice. We analyzed the differences in chromatin-accessible regions, which allowed us to identify the landscape of binding events, regulatory DNA sequences, and putative TFs that are likely responsible for these alterations.

Animal model
Male APPswe/PS1dE9 mice, 8 months of age (n = 3), and male, age-matched C57BL/6 mice (n = 3) were purchased from Nanjing Biomedical Research Institute of Nanjing University (Nanjing, China). All animals were housed in a specific pathogen-free room under a 12-h light-dark cycle with ad libitum access to food and water. Mice were acclimatized for a week, after which they were euthanized by cervical dislocation, and hippocampal tissues were collected. All animal procedures were performed according to the criteria outlined in the Guide for the Care and Use of Laboratory Animals (National Institutes of Health, Bethesda, MD) and with approval of the Animal Care and Use Committee of Xuanwu Hospital of Capital Medical University, China.
Nuclei isolation, transposition reaction, and ATAC-seq library preparation Nuclei were isolated from frozen hippocampal tissues. In brief, frozen hippocampal tissue (50-60 mg) was homogenized with a mortar and pestle on ice. The homogenized samples were placed in 1.0 mL of chilled lysis solution, mixed gently, and incubated on ice for 20 min. The mixture was then filtered through a cell-strainer (BD Biosciences, Franklin Lakes, NJ), mixed with 1.8 mL 1.8 M sucrose buffer, and loaded on the surface of a 1.0 mL 1.8 M sucrose cushion buffer. The nuclei were centrifuged at 27,550×g at 4°C for 45 min and the pellets were resuspended in 0.5 mL chilled nuclei storage buffer and centrifuged at 500g at 4°C for 5 min. The quality of the prepared nuclei was assessed with a light microscope. After the nuclei were counted, 50,000 nuclei were prepared for transposition reactions. The transposition reactions and ATAC-seq library preparation generally followed the steps set out in the original ATAC-seq paper using Nextera DNA Library Preparation Kit (Illumina, San Diego, CA) [22]. After transposition, DNA was purified with the MinElute PCR Purification Kit (Qiagen, Valencia, CA) and eluted in 10 μL elution buffer. The amplified libraries were purified with Ampure XP beads (Beckman Coulter) and were quantified with the Qubit dsDNA High Sensitivity Assay (Invitrogen, Carlsbad, CA) in combination with the High Sensitivity DNA Assay (Agilent, Santa Clara, CA) on the Agilent 2100 Bioanalyzer.
ATAC-seq sequencing, library quality control, and data analysis Each ATAC-seq library was sequenced on the Illumina Hiseq PE150 sequencer to obtain 80-100 million of 2 × 150 bp paired-end reads per sample. The quality of sequencing data was evaluated with FastQC (v.0.11.7, http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc) and reads with Phred quality score > 30 were used for further analysis. Removing and trimming the adaptor sequences were performed to obtain clean data. The clean reads were then aligned to mouse genome mm10 with Burrows-Wheeler Aligner (0.7.10) [23]. Picard Tools (v.2.2.4, http://broadinstitute.github.io/ picard) was used for duplicate removal. Samtools (v.1.3.1) was performed to filter multiply mapped reads, and BED tools were used for filtering out mitochondrial reads [24,25]. Aligned reads from APP/PS1 mouse hippocampal tissues (8 months old, n = 3) or normal hippocampal samples from aged-matched control mice (n = 3) were merged and peak calling was conducted using MACS2 (v.2.1) [26]. Peaks that overlapped blacklisted regions were removed. The resulting sets of ATAC-seq peaks were considered as high confidence Tn5 hypersensitive site (THSS) regions. The annotation of THSS regions to genomic features was performed using the HOMER suite tool annotate-Peaks (v.3.12) [27]. For the ACAT-seq enrichment analysis, we employed BEDTools (v.2.25.0) to obtain the count of reads overlapping with known transcription start sites (TSSs), promoters, exons, introns, etc. We considered the translation start sites as the reference point and − 1 Kb upstream of the translation start codon ATG as the putative promoter region. Differential accessibility analysis was conducted using DEseq2 (v.1.4.5) [28]. This analysis takes all the ATAC-seq peaks called in APP/PS1 mice and control mice and detects for normalized read count differences at the peak region. Motif discovery at chromatin-accessible regions was performed using the default setting in the HOMER suite function findMo-tifsGenome.pl tool [27]. Tracks were extracted and visualized using the UCSC genome browser [29].
Heatmaps showing ATAC-seq enrichment at promoters were built using the R statistical package [30].

RNA-seq profiling and differential gene expression analysis
Total RNA was extracted using an RNeasy mini extraction kit (Qiagen), and RNA integrity was quantified by the Agilent RNA 6000 Nano Kit (Agilent) using the Agilent Bioanalyzer. Purified RNAs were reverse transcribed, and the resulting cDNA was further tagmented and PCR-amplified using the Nextera XT DNA sample kit (Illumina) to add the sequencing adaptors. The libraries were pooled and sequenced on the Illumina Hiseq PE150 sequencer to an average depth of 70 million reads per sample. The quality control of sequencing was performed via FastQC (v.0.11.7, http://www.bioin formatics.bbsrc.ac.uk/projects/fastqc).
Reads were aligned to mm10 using the STAR splice-aware aligner (v.2.5.2b). QoRTs (v.1.0.7) were used to gather read counts per sample per gene for differential gene expression analysis [31]. Normalized RPKM (reads per kilo base million reads) values were used as a measure of gene expression. Differential gene expression analysis was performed using DESeq2 (5% FDR). The heatmap of RNA expression was produced using the correlation of normalized gene-level FPKM (fragments per kilobase million reads) values across samples with the heatmap.

Chromatin accessibility and gene expression correlation
For purposes of assessing the global relationship between the ATAC-seq signal at promoters and the transcription levels of the corresponding genes, genes were categorized into high, medium, and low groups, based on their mRNA levels according to the RNA-seq data. A threshold value was determined by dividing the mRNA values (RPKM normalized values) in three quantile groups according to their means using the R statistical package. Violin plots were produced using 'ggplot2' from the R package. For comparison and visualization, ATAC-seq and RNA-seq enrichment data at various genomic features are shown on a log2 scale. In addition, the 25 most highly expressed genes and the 25 least highly expressed genes in APP/PS1 mice and controls were identified, and their transcriptional start sites (Ensembl) were extracted from the UCSC Table  Browser. Regions expanded to 100 kb surrounding these TSSs were intersected and overlapped with the ATACseq libraries, then quantified and plotted, and the ATAC-seq signal over the most and least highly expressed genes was also quantified and plotted. In addition, the top 50 altered (highest and lowest) ATACseq peaks were extracted by q value, and the expression of the nearest gene was quantified and plotted as a final metric to relate the RNA-seq and ATAC-seq datasets.

Gene ontology analysis
Gene ontology (GO) analysis was performed to facilitate elucidating the biological implications of unique genes in the significant profiles of the gene. We annotated the genes associated with the accessible chromatin regions using the Genomic Regions Enrichment of Annotations Tool (GREAT, v.3.0.0) [32]. The annotated genes were then analyzed based on the GO annotations in the database to obtain all the GO involved genes. The significant level of each GO was calculated by the Fisher test. P values < 0.05 were considered significant.

Pathway analysis
Pathway analysis was performed to explore the annotated genes or differential genes enriched signaling pathways. The annotated genes or differential genes were analyzed based on KEGG database to obtain all the involved pathway terms. The significant level of each pathway term was calculated by the Fisher test. P values < 0.05 were considered significant.

Statistical analyses
Data are expressed as mean ± SD. Comparison between two groups was conducted with Student's t test. Differences between groups were considered to be statistically significant at P < 0.05. Statistical analyses were performed using the GraphPad Prism 6 software (GraphPad, La Jolla, CA).

Results
Chromatin accessibility by ATAC-seq is predictive of active transcription in APP/PS1 mice As APP/PS1 mouse is a widely used animal model to study Alzheimer's disease [33], we employed hippocampal tissues of APP/PS1 mice and age-matched wild type (WT) mice to generate ATAC-seq libraries. For each group, we obtained three independent ATAC-seq replicates. The similarity among replicates is high compared to the similarity between groups (Supplementary Figure  S1). The chromatin was fragmented by Tn5 transposase into nucleosome-free, mono-nucleosome, and dinucleosome patterns, and the similar distribution of fragment sizes suggested that chromatin is accessible to Tn5 transposase to the same degree in all samples independently between different groups (Fig. 1a). Distance analysis of ATAC-seq data showed that the location of THSS regions was mainly focused in a window of 1 kb upstream from the translation start codon (ATG) and was scarce at distances greater than 2 kb of the nearest gene (Fig. 1b). In addition, we found the ATAC-seq signal is significantly enriched at − 1Kb~+ 1Kb from TSSs (Fig. 1c). Based on the above findings, we annotated THSSs as promoters if the peak is located less than 1 kb upstream from the corresponding ATG (Fig. 1d) and used this annotation for the following analysis. The genomic location distribution of peaks showed that the peaks distributed on the promoter-TSS regions of APP/PS1 mice were significantly more than those of WT mice (26.66 ± 1.050% vs 19.14 ± 1.449%), revealing that the accessibility of chromatin around the TSS regions of APP/PS1 mice was higher than that of WT mice (Fig. 1e, f).
AD-associated metabolism involves numerous genes that participate in multiple signaling pathways. To investigate the functional role of the chromatin-accessible regions in the regulation of gene expression involved in signaling pathways and gene function, we analyzed different pathways and biological processes in AD model mice and WT mice. Our results showed that 204 annotated genes were associated with chromatin-accessible regions in AD mice and were involved in active signaling pathways. These regions were enriched in different signaling pathways including the PI3K-Akt, Hippo, TGFβ, and Jak-Stat signaling pathway that play essential roles in regulating of cell proliferation, cell apoptosis, and inflammatory responses (Fig. 2a, Supplementary Table  S2). In consideration that neuronal death and microglia proliferation increase in AD mice, they may account for  some of changes of signaling pathway above in AD [34][35][36][37][38]. Meanwhile, 237 annotated genes were found to associate with chromatin accessibility in decreased signaling pathways including RAS signaling pathway, glutamatergic synapse, and glycosaminoglycan biosynthesis (Fig. 2a, Supplementary Table S3). GO analysis showed that several AD-associated biological processes were reduced including cellular response to hyperoxia and insulin stimulus, synaptic transmission, and positive regulation of autophagy (Fig. 2b, Supplementary Table S4), while ventricular septum morphogenesis, negative regulation of protein ubiquitination, and protein homooligomerization were induced in AD-associated biological processes (Fig. 2b, Supplementary Table S5). In addition, some critical cellular components were deficient in the hippocampus of AD mice, such as synapse, postsynaptic membrane, dendrite, axon, neuronal cell body, etc (Fig. 2c, Supplementary Table S6), and the increased cell components were also identified (Fig. 2c, Supplementary Table  S7).

Differential chromatin accessibility in APP/PS1 mice associated with gene expression
To explore the relationship between chromatin accessibility and transcription genome-wide, we performed RNA-seq and integrated with ATAC-seq data that computed the ATAC-seq signal level at known TSSs in groups of genes categorized with low, medium, and high mRNA level. Results revealed that the level of enrichment in ATAC-seq signal at the TSS region positively correlated with mRNA abundance of the annotated gene (Fig. 3a), indicating that highly transcribed genes showed a more open chromatin landscape than genes with low transcriptional level. Moreover, we found that the gene expression differed significantly between AD model mice and WT mice. We examined 1690 increased ADassociated chromatin-accessible regions and 1003 decreased AD-associated chromatin-accessible regions from ATAC-seq data. Meanwhile, a total of 1090 upregulated and 1081 downregulated genes were identified in AD model mice from RNA-seq data (Fig. 3b). In addition, we detected 740 genes associated with an increased ATAC-seq signal, of which 43 exhibited upregulated mRNA levels, and 722 were associated with decreased ATAC-seq signal, of these, 44 genes displayed downregulated mRNA levels (Fig. 3c, Supplementary  Table S8, Supplementary Table S9). Furthermore, the region around high expression genes in the AD hippocampus, such as Prnp, Olfr31, and Ifi44, had gained open chromatin architecture, whereas regions around low expression genes, for example Cox8b, Selenov, and Pax7, showed lost chromatin signals (Fig. 3d, e).
The heatmap of RNA-seq is shown in Fig. 4a, and the 50 most variable genes in AD mice are listed. The APP gene, which encodes the amyloid protein precursor, was also found upregulated in AD mice (Supplementary  Table S10). Furthermore, we observed the chromatin accessibility of cis-regulatory elements in the APP gene. The results showed that the locus containing the upstream APP enhancer exhibited higher chromatin accessibility (Fig. 4b). These results indicate that the AD-associated open chromatin regions play a functional role in AD-specific transcriptome aberrations. In addition, the upregulated differential genes expressed in AD mice were enriched in the MAPK signaling pathway, osteoclast differentiation, estrogen signaling pathway, and neuroactive ligand-receptor interaction (Fig. 5a). Meanwhile, the downregulated differential genes expressed in AD mice were enriched in metabolic pathways, valine, leucine, and isoleucine degradation and fatty acid degradation (Fig. 5b). Moreover, the GO analysis showed that the upregulated genes in AD were enriched in nervous system development, histone H3-K4 methylation, and glial cell development (Fig. 5c), and downregulated genes in AD were enriched in oxidationreduction process, ion transport, and lipid metabolic process (Fig. 5d).

Chromatin accessibility footprints identify critical transcription factors in AD mice
To elucidate potential mechanisms of the relationship between over-expression genes and open chromatin regions, we investigated DNA transcription factor binding at AD-associated chromatin-accessible sites. The 50 most differential peaks of ATAC-seq in AD model mice and WT mice are shown in Fig. 6a. Moreover, we identified 15 most enriched transcriptional factor motifs in the increased chromatin-accessible regions in AD (Fig. 6b). Among the top 15 enriched motifs, those of Olig2, NeuroG2, NeuroD1, Atoh1, and TCF4 have been demonstrated to play key roles in AD. Furthermore, the TCF4 motif was identified in the open chromatin region around the TSS in AD-specific genes such as APP, GSAP, and SORL1 (Fig. 6c). In addition, motif enrichment for regions of decreased chromatin accessibility in AD is showed in Supplementary Figure S2.

Histone H3K4me3 and H3K27ac marks are enriched in open chromatin regions in AD model mice
It is well established that active histones are important marks for gene regulation, as H3K4me3 and H3K27ac are characteristic of TSS/promoter region and are associated with TF binding [39][40][41]. Thus, we investigated the histone modifications in open chromatin regions of the most upregulated genes in AD mice, including Sele, Ccr6, CD300lf, Clec7a, and Cst7. The results showed that four of the five most upregulated genes, except for CD300lf, displayed a higher level for both H3K4me3 and H3K27ac marks in chromatin-accessible gene regions in the hippocampus of AD model mice than in WT mice. Notably, the level of H3K27ac in the Cst7 gene and the levels of H3K4me3 and H3K27ac in the Ccr6 gene exhibited more than fivefold increases (Fig. 7). These results indicate that H3K4me3 and H3K27ac marks correlating positively with chromatin accessibility regulate gene expression.

Discussion
The genome-wide landscape of AD has been studied widely, and the gene expression patterns involved in in the process of AD have been described in detail [42,43]. However, the mechanism of functional genome alterations in AD requires further investigation. Recent advances in chromatin profiling, especially genome-wide chromatin accessibility profiling methodologies, including MNase-seq, DNase-seq, FAIRE-seq, and ATAC-seq, have made it possible to analyze the alterations in the accessible chromatin state and allow us to better understand the molecular mechanisms of numerous diseases such as cancer, malaria, and metabolic disorders [44][45][46][47]. Although DNase-seq and MNase-seq can provide some subsets of the information obtained by ATAC-seq, they still have some limitations, for example large cell numbers, longer experimental time, and limited applicability to many systems. ATAC-seq offers substantial advantages over existing technologies due to its speed, simplicity, and low input cell number requirement [48]. In the present study, we performed ATACseq to examine the chromatin accessibility in AD model mice, demonstrated significant alterations in chromatin accessibility, and described transcriptional profiles in AD hippocampal samples.
Our results showed that the most accessible regions localize around the TSS and that open chromatin at these sites is predictive of active transcription, in agreement with the pattern reported in previous studies [46]. Given that recent large-scale association studies suggested genes related to AD risk are involved in many different biological pathways [42], we analyzed different biological processes and pathways in AD model mice and WT controls. Among the signaling pathways, we found to be strongly associated with AD were the Hippo and TGF-β, known to induce cell apoptosis [49,50]. Regarding Hippo signaling, a recent study reported that the precursor of Aβ can promote the nuclear translocation of Foxo3a by inducing MST1-dependent phosphorylation of Foxo3a. The MST-Foxo pathway is considered a branch of the Hippo pathway; it activates a proapoptotic member of the Bcl-2 family triggering an intrinsic apoptotic pathway, thus resulting in neuronal death [51]. Relevant to TGF-β signaling, different studies have shown increased expression of TGF-β1 and TGF-β2 in brains of individuals with AD [52][53][54]. Given the extensive evidence of microglial dysfunction in neurodegeneration, including changes in microgial phagocytic activity, it is possible that changes in brain TGF-β signaling in AD could alter microglial state and trigger their pathogenic functions [55].
We also found the Jak-Stat and PI3K/Akt signaling pathways to strongly associate with AD. Several studies have demonstrated the Jak-Stat signaling as a possible underlying pathogenetic mechanism of AD, showing the importance of inflammatory pathways involved in AD  [56,57]. Besides, the PI3K/Akt pathway has been found to be related to tau protein hyper-phosphorylation [58]. The alterations of the chromatin accessibility in these signaling pathways may partly elucidate the mechanism of pathological changes in AD such as neurofibrillary tangles induced by phosphorylation of tau protein, the upregulation of inflammation, and neuronal apoptosis.
To observe the differential chromatin accessibility in APP/PS1 mice associated with gene expression, we examined 740 chromatin-accessible regions in AD with 43 upregulated associated genes and identified several genes with a notable increasing expression, including Sele, Clec7a, Cst7, Ccr6, and CD300lf, which may have profound significance in the AD process. The Sele gene encodes a cell-surface glycoprotein E-selectin that has a role in immunoadhesion [59]. It is thought to be responsible for the accumulation of leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. Li et al. reported that E-selectin in CSF was significantly elevated in clinically diagnosed AD patients without the typical AD CSF biomarker signature compared to those with a positive biomarker signature [60]. Clec7a and Cst7 play key roles in immune regulation. Recent studies have demonstrated that Clec7a and Cst7 are upregulated in the switch from homeostatic microglia to disease-associated microglia, which is found in AD at the proximity of the Aβ plaques [61,62]. Additionally, highly significant increases of Ccr6 expression were observed in the brain and spleen of both the . c Upregulated gene enrichment in biological process (20 most enrichment biological process are listed). d Downregulated gene enrichment in biological process (20 most enrichment biological process are listed). The rich factor is defined as the ratio of the number of differential genes enriched in the pathway to the number of annotated genes younger and older 3 × Tg-AD mice, implicating Ccr6 may be a possible biomarker for the development of AD-like disease [63]. On the other hand, we also identified enriched motifs at chromatin-accessible regions in AD, for example motifs for Olig2, NeuroG2, NeuroD1, Atoh1, and TCF4. Several of the most upregulated genes and AD-specific genes, we identified possess the enriched motif binding sites around the chromatin-accessible regions of TSS, indicating that the motifs enriched in open chromatin regions may play important roles in regulating the gene expression that participates in the AD process.
Accessibility at regulatory regions modulated by chromatin remodeling processes involves not only nucleosome occupancy, but also histone modifications, especially H3K4me3 and H3K27ac, which are necessary for transcription factor binding. Therefore, we investigated these histone modifications in the c Tracks for AD sample at the APP, GSAP, and SORL1 genes with predicted TCF4 binding sites. The predicted TCF4 binding sites are shown with orange bars on the upper areas of the tracks. The TSS is indicated by blue arrow upregulated genes in AD. We found that both H3K4me3 and H3K27ac marks are highly expressed in the promoter regions of Sele, Clec7a, Cst7, and Ccr6 genes. A recent study has reported the patterns of H3K27ac in entorhinal cortex samples from AD cases and identified several H3K27ac-associated transcriptional variation genes, suggesting that histone marks may indeed be critical events causing activation of genes favoring the progression in AD [64].

Conclusion
In summary, our work provides a novel strategy to study transcriptional regulation in AD through the description of open chromatin profiling by ATAC-seq. The utilization of this technique in AD allows us to identify the regulatory elements that play critical roles in the AD process. Furthermore, our data present an association between TF binding, chromatin-accessible regions, and gene regulation, providing new insights into the molecular mechanisms of AD. However, the future challenge is to elucidate how the alteration of TF influences the malfunctioning of critical genes involved in AD pathogenesis.