Fig. 3
Effect of repeated antibody injections in wt mice. A Study design: 4-month-old wt mice, subjected to repeated antibody injections, were injected with an iodinated variant of the same antibody (diagnostic dose), followed by extraction of blood, brain, and organs. B Blood pharmacokinetcs after 1st, 2nd, and 3rd injection of [125I]RmAb158-scFv8D3 in wt mice. C ELISA measuring ADA in plasma from mice in B in comparison with naïve (untreated) and RmAb158 treated mice, using RmAb158-scFv8D3 as both capture and detecting antibody. D Sagittal half-body PET images obtained during 0–10 min after injection of [124I]RmAb158-scFv8D3mut 3 in wt mice with or without pretreatment with the same antibody. Yellow arrow points to the spleen, which was readily visible in naïve control mouse. Blood, brain, liver, and spleen radioactivity concentration 1 h after injection of radiolabeled RmAb158-scFv8D3mut 3 (E), mouse chimeric 8D3 IgG (F), aggregate free RmAb158-scFv8D3 (G), or tandem di-scFv3D6-8D3 (H) in untreated mice (blue) or after repeated injections of the same antibody (red). A was created with BioRender