Entorhinal cortex epigenome-wide association study highlights four novel loci showing differential methylation in Alzheimer’s disease

Table 4 Results of EWAS meta-analysis using Braak staging

CpG	Position	Genes	p-value	Effect (β)	Previous Studies (ref.)^a
cg03169557	chr16:89,598,950	RPL13, SPG7	1.03E-09	0.0042	[8, 16,17,18]
cg05066959	chr8:41,519,308	NKX6-3, ANK1, MIR486	2.76E-09	0.0092	[6, 8, 9, 16,17,18]
cg20618448	chr19:49,962,324	FLT3LG, ALDH16A1	1.42E-08	0.0043	[18]
cg05030077	chr16:2,255,199	MLST8	5.89E-08	-0.0026	[10]
cg05228284	chr19:2,720,847	DIRAS1	9.25E-08	-0.0023	-
cg05972352	chr13:113,663,373	MCF2L, F2	1.20E-07	0.0058	genes previously reported [8, 12, 16, 18]
cg14761246	chr3:182,968,758	MCF2L2, B3GNT5	1.55E-07	0.0039	[17]
cg22090150	chr17:4,098,227	ANKFY1, CYB5D2	1.57E-07	0.0048	[14, 17, 18]
cg07571519	chr10:73,472,315	C10orf105, SLC29A3	1.62E-07	0.0050	[9, 17]

Experiment-wide significant CpGs (p < 1.64E-07) in the meta-EWAS ascross three datasets (London-1, London-2, Oxford) using Braak stage
^aEvidence for implication of same or largely overlapping locus from studies using DNAm assessments in AD-related phenotypes. Annotation of CpGs to specific gene regions was based on the Illumina manifest (v1.0 B5) for the EPIC array and the GREAT annotation tool [28]

ISSN: 1758-9193