Fig. 3

Region-of-interest (ROI)-based analysis. Average signal intensity changes over time reflecting Gd-DOTA contrast agent distribution in the OB (A), the HC (B), the aqueduct (C), the medulla (D), the pons (E) and the CB (F) for the CTL (cyan) and the AD (red) groups of mice. The grey rectangle in each panel represents the pre-acquisition time (acquisition started at 30 min post-Gd-DOTA infusion) with zero indicating the time of injection. An overview of the brain location of each ROI can be found in the top right corner of each panel (blue rectangles). OB, olfactory bulb; HC, hippocampus; CB, cerebellum; N, ROI size in voxels; *significant difference in the area under the curve (AUC) across the two groups indicates a differential distribution of contrast agent (N = 5 mice per group; two-sample t-test; p < 0.05; see the ‘Methods’ section)