Fig. 4From: A novel human tau knock-in mouse model reveals interaction of Abeta and human tau under progressing cerebral amyloidosis in 5xFAD miceAnalysis of tau and Aβ pathology. a,b Aβ staining using 3A1 antibody in representative sagittal brain slices of female, 13-month-old 5xFAD (a) and 5xFADxhtau-KI (b) mice. Scale 500 μm. c Quantification of the occupied total plaque area in brain slices analogous to a and b in female (F) and male (M) 13-month-old 5xFAD and 5xFADxhtau-KI mice. Plaque area in WT and htau-KI mice was equal to zero (not shown). Mean±SEM. n=5–8. * P < 0.05 unpaired t-test. d Sections from the hypothalamic region of 13-month-old, female 5xFAD and 5xFADxhtau-KI mice showing Congo red-stained Aβ plaques surrounded by dystrophic neurites (arrow heads). Three different antibodies were used for DAB staining of tau protein: CP13 (pathologic tau), PHF1 (later stage tangles), and MC1 (conformation-specific). Note that MC1-positive tau occurs exclusively in 5xFADxhtau-KI. Scale 20 μm. e Quantification of total and MC1-surrounded plaques in the hypothalamus of female 5xFAD and 5xFADxhtau-KI mice of 7 months (7 M) and 13 months (13 M) of age. Mean ± SD. 5xFAD, 7 M (n=9); 5xFAD, 13 M (n=6); 5xFADxhtau-KI, 7 M (n=7); 5xFADxhtau-KI, 7 M (n=6). f Quantification of total and MC1-surrounded plaques in the inferior colliculus of female 5xFAD and 5xFADxhtau-KI mice of 7 months (7 M) and 13 months (13 M) of age. Mean ± SD. 5xFAD, 7 M (n=8); 5xFAD, 13 M (n=5); 5xFADxhtau-KI, 7 M (n=7); 5xFADxhtau-KI, 7 M (n=6)Back to article page