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Fig. 2 | Alzheimer's Research & Therapy

Fig. 2

From: A novel human tau knock-in mouse model reveals interaction of Abeta and human tau under progressing cerebral amyloidosis in 5xFAD mice

Fig. 2

Analysis of tau isoform expression in htau-KI mice and generated crossbreds. a, b Absolute quantification of 3R tau (a) or 4R tau (b) based on a standard curve generated from a human 3R tau- or 4R tau-specific DNA sequence, respectively. Depicted are the amounts of 3R or 4R tau mRNA in molecules/ng RNA from male (M) and female (F) WT, 5xFAD, htau-KI and 5xFADxhtau-KI mice at the ages of 7 and 13 months. Note, no PCR product was obtained for WT and 5xFAD for 3R tau. Mean±SEM, n = 2–3 per group. *P < 0.05, one-way ANOVA, Tukey’s multiple comparison test. c Combined scatter and bar plot (mean±SD) for illustration of the 3R/4R tau mRNA ratio in htau-KI and 5xFADxhtau-KI mice depending on sex and age (7 and 13 months). Shown is a summary of 2 independent experiments. n = 4–6. d Combined scatter and bar plot (mean±SD) for illustration of the 3R/4R tau protein ratio in htau-KI and 5xFADxhtau-KI mice depending on sex and age (7 and 13 months). n = 5. e Western blots stained with 3R- and 4R-specific antibodies in male (M) and female (F) htau-KI mice at the ages of 3–12 months in comparison to the reference protein GADPH. WT mice only express 4R tau isoforms. Tau isoforms are labeled on the right. Het = heterozygous htau-KI mouse, xM = age in months. Uncropped image files can be found as Additional files 12, 13 and 14. f Immunohistochemical staining using 3R- and 4R-specific antibodies. Depicted are representative images of the hippocampal structure from female WT, 5xFAD, htau-KI, and 5xFADxhtau-KI mice at 13 months of age. Note the presence of 3R tau in pyramidal neurons in htau-KI and 5xFADxhtau-KI mice. The staining is representative of all sexes. Scale bar 500 μm

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