Fig. 6

Effect of immunodepletion on the neurotoxicity of AD brain extracts. Primary mouse neurons were treated for 24 h with artificial cerebrospinal fluid (aCSF), extracts of non-AD controls (grey) or extracts of AD patients (black) that were either untreated (-) or immunodepleted with ALZ-201, 4G8 or IgG3. High-content automated microscopy was employed for the quantification of the number of A neuronal nuclei: B morphology (as determined by segments, branches and extremities), and C number of vGlut1-positive presynapses. Morphological and synaptic parameters were normalised against the number of neurons. Values are displayed as the % change to cultures treated with aCSF. Bar graphs show the mean ± SD and data points represent the individual values for control (grey) and (immunodepleted) AD brain extracts (black). Shapiro-Wilk normality testing was followed by parametric (ordinary) or non-parametric (Kruskall-Wallis) one-way ANOVA, and groups were compared to pre-immunodepleted conditions using Dunn’s or Dunnet’s post hoc test. Significance values: (B: segments) AD vs Ctrl <0.0001, vs 4G8 <0.0001, vs ALZ-201 =0.006; (B: branches) AD vs Ctrl = 0.0008, vs 4G8 <0.0001, vs ALZ-201 <0.0001; (B: extremities) AD vs Ctrl = 0.0053, vs 4G8 = 0.0004, vs ALZ-201 = 0.0145; (C: presynapses) AD vs Ctrl <0.0001, vs 4G8 <0.0001, vs ALZ-201 = 0.0011. ns = not significant