Fig. 7

Concentration of Aβ in spun TBS and TBS-T extracts from the brain of tg-ArcSwe mice following treatment with therapeutic doses of 30 nmol/kg body weight of sNEP-scFc-scFv8D3 or muNEP-scFc-scFv8D3, using scFc-scFv8D3 as the negative control. A Significantly lower concentration of Aβ monomers (p value 0.03) was detected in the TBS-T brain extracts of the sNEP-scFc-scFv8D3-treated group compared to the scFc-scFv8D3 control group using a m266-3D6 ELISA. B Also, the levels of oligomers with a hairpin were significantly lower after treatment detected with a A11-3D6 ELISA. C Using an ELISA with one antibody detecting the N-terminal (3D6) and one detecting the C-terminal of Aβ, i.e. an ELISA that will detect aggregates with no hidden terminal and monomers, no significant differences were detected. D ELISA using the monoclonal antibody 3D6 as both capture and detection which will exclude the detection of monomers and will also allow the detection of aggregates that have the C-terminal hidden. E m266-Aβ42 ELISA. F 3D6-Aβ42 ELISA. G mAb158-3D6 ELISA. H ELISA with the 3D6 antibody as capture and the arctic-specific mAb27 antibody as detection. I Murine Aβ-3D6 ELISA. Results are presented as mean ± SD. One-way ANOVA with Bonferroni’s multiple comparison test was applied (n = 4/sNEP-scFc-scFv8D3 and muNEP-scFc-scFv8D3; n = 3/scFc-scFv8D3) (p > 0.05 = ns; p ≤ 0.05 = *; p ≤ 0.01 = **; p ≤ 0.001 = ***). Standard curves were used when possible. In cases of detection of a mixture of aggregates and monomers, no standard curve would be able to correctly correspond to the levels measured in homogenates and therefore the A450 measured is given, which cannot be compared between different ELISAs