Fig. 2From: Blood–brain barrier penetrating neprilysin degrades monomeric amyloid-beta in a mouse model of Alzheimer’s diseasePurity analysis and structural stability of the recombinant proteins. A SDS-PAGE showing a strong band at around 160 kDa for sNEP-scFc-scFv8D3 and muNEP-scFc-scFv8D3 and a single band at around 80 kDa for scFc-scFv8D3. IgG2c isotype antibody of 150 kDa was loaded as a control. The complete SDS-PAGE gel can be seen in Supplementary Fig. 6. B Structural stability of the recombinant proteins under thermal stress measured with Tycho nt.6 system. The following inflection temperatures were detected for the proteins (58 °C for sNEP; 58 and 79 °C for sNEP-scFc-scFv8D3; 58 and 79 °C for muNEP-scFc-scFv8D3; 72 and 79 °C for scFc-scFv8D3)Back to article page