Table 1 Overview of primary antibodies employed
From: Granulovacuolar degeneration bodies are independently induced by tau and α-synuclein pathology
Antibody | Phospho epitope | Species | Dilution | Source | Product number | ||
|---|---|---|---|---|---|---|---|
Tissue | In vitro | ||||||
IF | IHC | ||||||
AT8 (p-tau) | Ser202/Thr205 | Mouse monoclonal | 1:800 | 1:200 | 1:400 | Thermo-Scientific | MN1020 |
AT100 (p-tau) | Thr212/Ser214 | Mouse monoclonal | 1:200 | Thermo-Scientific | MN1060 | ||
CK1δ | Mouse monoclonal | 1:250 | 1:250 | 1:1000 | Santa Cruz Biotechnology | sc-55553 | |
CK1δ | Rabbit polyclonal | 1:250 | 1:400 | 1:500 | Thermo-Scientific | PA5-32129 | |
LIMP2 | Rabbit polyclonal | 1:300 | 1:500 | Novus Biologicals | NB400-129 | ||
MAP2 | Chicken polyclonal | 1:100 | 1:250 | Abcam | Ab5392 | ||
MC1 | Mouse monoclonal | 1:500 | Kind gift from Dr. Peter Davies | ||||
pPERK | Thr981 | Rabbit polyclonal | 1:800 | 1:1000 | Santa Cruz Biotechnology | sc-32577 | |
p-αsyn129 (p-α-syn) | Ser129 | Mouse monoclonal | 1:100/1:500 | 1:100 | 1:2500 | Merck | MABN826 |
p-tau217 (p-tau) | Thr217 | Rabbit polyclonal | 1:1600 | 1:100 | Thermo-Scientific | 44-744 | |