Fig. 6

Pathological α-synuclein accumulation induces GVBs in the absence of pathological tau. a Representative confocal images of immunofluorescence staining for MC1 (green) and pPERK (magenta). Separate channels are in greyscale and MAP2 is shown in grey in the merged images. FTDtau¹⁺²-transduced neurons were used to set MC1 imaging settings for the detection of pathological tau and are shown as a reference. MC1 and merged images are also shown at increased intensity to visualise low abundant pathological tau. b, c Single-cell quantification of pathological tau load in GVB− and GVB+ neurons in the α-syn pathology model. b Representative confocal images of primary mouse neurons treated with α-syn PFFs to induce α-syn pathology or the seed vehicle as control. Immunofluorescence staining was performed for AT8 (green) and pPERK (magenta). Separate channels are shown in greyscale and merged images show neurons in grey and nuclei in blue. FTDtau¹⁺²-transduced neurons were used to set AT8 imaging settings for the detection of pathological tau and are shown as a reference. c Single-cell quantification of somatic tau load in GVB− (blue) and GVB+ (pink) neurons in the α-syn model represented as the Log2-transformed corrected mean AT8 intensity. Vehicle-treated control neurons are shown in grey. N=3 independent experiments, n=64, 17 and 123 for control, α-syn PFFs/GVB+ and α-syn PFFs/GVB− populations, respectively. n.s. not significant, nested one-way ANOVA with Tukey’s post hoc test