Skip to main content
Fig. 2 | Alzheimer's Research & Therapy

Fig. 2

From: Granulovacuolar degeneration bodies are independently induced by tau and α-synuclein pathology

Fig. 2

Pathological tau accumulation precedes GVB formation in the FTDtau1+2 model. a Schematic representation of experimental set-up to assess the temporal sequence of tau pathology and GVB formation. Plating and fixation day were the same as the regular protocol (Fig. 1a), whereas transduction with FTDtau1+2 was at different time points, resulting in different durations of exposure. b Quantification of the mean MC1 immunofluorescence intensity (green) and the number of GVB+ neurons (pink) per time point, relative to 15 days FTDtau1+2 exposure (100%). N=3 independent experiments, n=6 observations per time point, containing data from 5037–5775 neurons, were analysed. Data points represent the corrected mean value per well. Error bars represent the standard error of the mean (SEM). **p < 0.01; ***p < 0.001; ****p < 0.0001, compared to untransduced control (0 days), nested one-way ANOVA and Dunnett’s post hoc test. c Representative epifluorescence images for selected time points. Immunofluorescence staining was performed for the neuron-specific dendritic marker MAP2 (grey), MC1 (green) and CK1δ (magenta). Nuclei are visualised with DAPI (blue). Red arrowheads point to GVB+ neurons

Back to article page