Fig. 3
From: Apolipoprotein E imbalance in the cerebrospinal fluid of Alzheimer’s disease patients
Characterization of CSF apoE species under reducing, non-reducing, and native conditions. A, B Control (Ct) and AD CSF samples immunoblotted under reducing (R) or non-reducing (NR) SDS-PAGE with A apoE antibody (Ab178479) or B apoE4-specific antibody (NBP1-49529). Note that apoE4 is identified as part of the 100-kDa bands despite the inability to form disulfide-linked dimers. C Representative blot of native-PAGE studies, showing the apoE monomers and dimers. The discrepancy in molecular weight is likely due to the differences between native-PAGE and SDS-PAGE electrophoretic conditions which affect the migration of proteins. Rec, recombinant apoE3; Den CSF, denatured CSF. D Quantification of the ratio of apoE dimers compared to monomers across the different APOE genotypes, estimated by native-PAGE (see C). Scatter plots of apoE levels are represented. The graphs represent mean ± SEM, and the numbers below represent median ± SD. Significant p values are indicated