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Fig. 2 | Alzheimer's Research & Therapy

Fig. 2

From: Nanoscale alterations in GABAB receptors and GIRK channel organization on the hippocampus of APP/PS1 mice

Fig. 2

Reduction of GIRK2 immunoparticles in presynaptic compartments of APP/PS1 mice. Electron micrographs showing presynaptic immunoparticles for GIRK2 in the stratum radiatum of the hippocampal CA1 field at 12 months of age, as detected using the SDS-FRL technique in wild type and APP/PS1 mice. A, B In wild type, GIRK2 immunoparticles were mostly found along the extrasynaptic site (blue arrows) of axon terminals (at). Although few immunoparticles were detected within the active zone (az, purple overlay), recognized by the concave shape of the P-face and the accumulation of IMPs, many were detected at the border between the active zone and extrasynaptic sites. In these compartments, GIRK2 immunoparticles were observed forming clusters (blue ellipses/circles) and scattered (blue arrows) outside the clusters. C In APP/PS1, fewer GIRK2 immunoparticles forming clusters or scattered (blue arrows), were detected at extrasynaptic sites of axon terminals (at), at the edge of the active zone (az), or within the active zone (red overlay). D Quantitative analysis showing that the density of GIRK2 immunoparticles was significantly reduced in the APP/PS1 mice compared to age-matched wild type controls in the two presynaptic compartments analysed (Mann–Whitney test, *p < 0.05; ***p<0.001). Error bars indicate SEM. Scale bars: AD, 0.2 μm

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