Fig. 2
Quantification of GM1-containing EVs Using the idICA. A Western blot analysis of CD9, ganglioside GM1, and ßIII tubulin in N2a cell lysates (1 × 105 cells/lane) and EVs (1 × 107 cells/lane). B Representative fluorescent images of various concentrations of N2a-derived EVs in the idICA, which is constructed from CTB capture and anti-CD9 detection. Each image shows a block of well array corresponding to 10,000 microwells. Scale bar, 200 μm. C, D The ratio of fluorescent beads to trapped beads in a block of well array is plotted as the concentration of CD9 captured on CTB-coated beads (CTB-CD9) in N2a-derived EVs. Plots on the semi-logarithmic (C) and linear (D) scales are shown. Data represent mean ± SD (n = 3 each)