Fig. 4
From: Distinct sex-specific DNA methylation differences in Alzheimer’s disease
Differential DNA methylation and gene expression at the PM20D1 gene in blood samples of male AD and cognitively normal subjects. We first removed effects of age, estimated proportions of immune cell types, and batch effects in both DNA methylation and gene expression data separately, by fitting linear regression models and extracting residuals. The results showed that A DNA methylation at chr1:205819088-205819609 in the promoter region of PM20D1 is hypomethylated in AD subjects, B PM20D1 gene expression levels are significantly up-regulated in AD subjects, and C there is a strong negative association between DNA methylation and gene expression at this locus. Abbreviations: dnam, DNA methylation; CN, cognitively normal; rlm, robust linear model