Fig. 10
From: Deletion of Abi3/Gngt2 influences age-progressive amyloid β and tau pathologies in distinctive ways
S209F mutation leads to altered phosphorylation in ABI3 protein. a HEK293T cells were transiently transfected with WT ABI3 or S209F ABI3 and separated on an immunoblot showing two distinct bands around 50 kDa. Higher bands that are as yet uncharacterized (marked by asterisk) are also denoted. The proteins were detected with two antibodies simultaneously using LiCor infrared dyes: anti-FLAG antibody (green) and anti S209-ABI3 antibody (red). b Lambda protein phosphatase assay performed at 30 °C on WT ABI3 and S209F ABI3 expressing HEK293T cell lysates detected with anti-FLAG antibody. c, d HEK293T cells were transiently transfected with WT ABI3 or ABI3 mutated at position 209 and separated on an immunoblot. The proteins were detected with two antibodies simultaneously using LiCor infrared dyes: anti-FLAG antibody (green) and anti S209-ABI3 antibody (red) (c) or anti-FLAG antibody (green) and anti N terminal ABI3 (Sigma) (red) (d). “Mock” denotes sham transfection procedure with no DNA. Data representative of at least two independent experimental replicates