Fig. 1

Schematic representation of steps during processing of plasma samples and the AD-seeds protein analyzer. To remove albumin, samples of plasma were incubated with 1 volume of albumin depletion reagent. After centrifugation, the pellet was resuspended directly in PBS, and then mixed with 5 μM Aβ40 and  20 μM ThT. Samples were subjected to a round of 130 cycles. The kinetics of fibril formation was monitored real time by the reading of the fluorescence intensity every 30 min using 440±10 nm excitation and 480±10 nm emission wavelengths. Aβ, β-amyloid; AD, Alzheimer’s disease; PBS, phosphate buffered saline