A novel D-amino acid peptide with therapeutic potential (ISAD1) inhibits aggregation of neurotoxic disease-relevant mutant Tau and prevents Tau toxicity in vitro

Table 1 Selected L-peptides from phage display selection against Tau^FL

Nr.	Sequence	Frequency	Inhibition of Tau^FL aggregation	Name
1	SVFKLSLTDAAS	1/80	+	ISAL1
2	NHDMDLLVWWMN	1/80	+/-	ISAL2
3	NWSMPGMTQGFL	13/80	-	ISAL3
4	DFHQRDDDSQQA	1/80	-	ISAL4
5	AMYQFSRNPHLP	3/80	-	ISAL5
6	VSPAWDARTRSA	2/80	-	ISAL6
7	MTPHGNSKTPSG	1/80	-	ISAL7
8	HDWYRSPRMGLF	1/80	-	ISAL8
9	DLSHGQDLMHHH	1/80	-	ISAL9
10	SASVTSKFDALL	-	-	ISAL1sam

The peptide sequences were determined after DNA sequencing of the positive phages. Each sequence was given a number in the list. (+/-) indicates comparably low inhibition of Tau^FL fibrillization, (-) indicates that the peptide showed no effect on fibril formation. The scrambled peptide (ISAL1sam) was synthesized and did not show any inhibition of Tau^FL fibrillization

ISSN: 1758-9193