Fig. 4

ISAD1 and ISAD1rev inhibit the aggregation of Tau^FL and Tau^RDΔK. A Fibrillization curves of Tau^FL in the presence of different concentrations of the peptide ISAD1 over a period of 24 h. The assay was performed using 5 μM Tau^FL with 1.25 μM heparin (H16K) and 10 μM ThT in HEPES buffer. ISAD1 was added in concentrations of 0.1 nM to 200 μM. B Dose-response curve of Tau^FL fibrillization process in the presence of ISAD1 and ISAD1rev (0.1 nM–200 μM). C 10 μM Tau^RDΔK in BES buffer was incubated with 2.5 μM heparin and 20 μM ThS to monitor fibrillization. D Dose-response curve of Tau^RDΔK in the presence of ISAD1 and ISAD1rev. The relative fluorescence of a buffer sample was subtracted. Fluorescence was measured at 520 nm in relative units (mean ± standard deviations of results, three replicates per run). The fluorescence of Tau^FL, Tau^RDΔK was set as 100% and the values and standard deviations of the other incubations are given as percentages of this maximum value. The data represent the mean values ± SD with n = 3 determinations