Fig. 10

ISAD1 and ISAD1rev reduce toxic effects of intracellularly expressed Tau^RDΔK aggregates. Expression of Tau^RDΔK in N2a cells was induced by adding 1 μg/ml doxycycline. The cells were incubated with different concentrations of ISAD1 and ISAD1rev (25, 50, 100, 250 μM) for 24 h at 37 °C followed by determination of MTT and LDH intensities. A Peptides prevent intracellular toxic Tau effects by preserving metabolic activity from 100 μM concentration (blue and red bars) similar to untreated cells (gray bar, UT -Dox) [n = 2; one-way ANOVA with Tukey’s post hoc test; F(11, 60) = 126; ***p ≤ 0.001]. B Protection of cell membrane integrity by D-peptides (blue and red bars) determined by LDH release [n = 2; one-way ANOVA with Tukey’s post hoc test; F(11, 60) = 256; ***p ≤ 0.001].