Fig. 6
From: The apolipoprotein receptor LRP3 compromises APP levels
LRP3 overexpression in CHO-PS70 cells decreases APP levels. a Representative immunofluorescence photomicrographs showing LRP3 and WGA labeling at the plasma membrane and discrete cytosolic areas of CHO-PS70 cells transfected with LRP3-flag cDNA. Nuclei were stained with the DNA dye Hoechst. b Western blots from CHO-PS70 cells transfected with LRP3-flag (CHO-LRP3) or pcDNA3.1 (CHO control) showing immunoprecipitation of APP (using a C-terminal APP antibody) and co-immunoprecipitation of APP (using an N-terminal APP antibody) and LRP3. T input, B bound fraction, U unbound fraction, IPc negative control. c qRT-PCR analysis showing expression of APP mRNA in CHO-PS70 transfected with LRP3-flag (LRP3) or pcDNA3.1 (Ctr, control). GAPDH was used as an internal control for mRNA expression (n = 13 for each condition). d Western blots and quantification of CHO-PS70 cells transfected with LRP3-flag (LRP3) or pcDNA3.1 (Ctr, CHO control) showing the expression in the cell extracts of LRP3, full-length APP, APP-CTF proteins, and tubulin, as an internal control (n = 6 for each condition, *p<0.001, t-test). d Western blots and quantification of CHO-PS70 cells transfected with LRP3-flag (LRP3) or pcDNA3.1 (Ctr, CHO control) showing the expression in the supernatants of sAPPα, sAPPβ, and Aβ, and the total protein as the internal control (n = 6 for each condition, *p<0.001, t-test)