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Fig. 1 | Alzheimer's Research & Therapy

Fig. 1

From: The apolipoprotein receptor LRP3 compromises APP levels

Fig. 1

ApoER2/reelin signaling upregulates LRP3 expression. a qRT-PCR analysis showing expression of LRP3 mRNA and LDLR mRNA after transfection with GFP cDNA (control) and full-length ApoER2 cDNA (fApoER2) in SH-SYSH cells. 18S was used as an internal control for mRNA expression (n = 10–12 for each condition, p < 0.001 for control versus fApoER2; t-test with Welch’s correction). Note that the X axis begins at 50%. b Quantification and western blot showing the expression of full-length ApoER2, ApoER2-CTF, and LRP3 proteins after fApoER2 transfection and reelin (12 μg/ml) treatment for 24 h in SH-SY5Y cells. Tubulin was used as an internal control (n = 9 for each condition, **p < 0.001 for expression of fApoER2, t-test with Welch’s correction, and ApoER2-CTF, t-test; *p < 0.05 for expression of LRP3, t-test). c Quantification and western blot showing the expression of LRP3 protein after reelin (12 μg/ml) treatment for 24 h or vehicle (Hanks’s media) in neuro-differentiated SH-SY5Y cells with retinoic acid (n = 9 for each condition, *p <0.05 t-test)

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