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Fig. 6 | Alzheimer's Research & Therapy

Fig. 6

From: Homozygous R136S mutation in PRNP gene causes inherited early onset prion disease

Fig. 6

Spontaneous generation of human misfolded recombinant proteins. Graphical representation of the emergence of spontaneous protein misfolding evaluated through Western blot analysis in SDS-PAGE for each round of recPMCA (outlined as R01–R15). Different 129M human recombinant proteins were grouped according to the substitution. Every experiment contained four tubes (intra-experimental duplicates) and was performed in duplicate as shown. The percentage of positive tubes (tubes showing a protease resistant signal after digestion with 80 mg/ml of PK) after each round of recPMCA was noted with different intensities of grey, as shown in the legend below the figure. Neither wild-type (wt) 129M not R136S 129M were able to misfold spontaneously. WT, wild type human rec-PrPSc

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