Fig. 6From: Homozygous R136S mutation in PRNP gene causes inherited early onset prion diseaseSpontaneous generation of human misfolded recombinant proteins. Graphical representation of the emergence of spontaneous protein misfolding evaluated through Western blot analysis in SDS-PAGE for each round of recPMCA (outlined as R01–R15). Different 129M human recombinant proteins were grouped according to the substitution. Every experiment contained four tubes (intra-experimental duplicates) and was performed in duplicate as shown. The percentage of positive tubes (tubes showing a protease resistant signal after digestion with 80 mg/ml of PK) after each round of recPMCA was noted with different intensities of grey, as shown in the legend below the figure. Neither wild-type (wt) 129M not R136S 129M were able to misfold spontaneously. WT, wild type human rec-PrPScBack to article page