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Fig. 1 | Alzheimer's Research & Therapy

Fig. 1

From: Activation of PLCβ1 enhances endocannabinoid mobilization to restore hippocampal spike-timing-dependent potentiation and contextual fear memory impaired by Alzheimer’s amyloidosis

Fig. 1

AβO disrupts synergistic enhancement of endocannabinoid (S-eCB) mobilization. a Schematic of the S-eCB mobilization protocol: whole-cell voltage-clamp recording in CA1 pyramidal cells (PCs) to measure Schaffer collateral (SC) stimulation-evoked inhibitory postsynaptic potentials (eIPSCs) in rat hippocampal slices in vitro. S-eCB mobilization was induced by postsynaptic CA1 PC spikes (spike, at 1 Hz) with DHPG (50 μM) for 60 s. b, c Time course of the changes in mean eIPSC amplitudes following DHPG (empty circle, n = 8, b), DHPG + AM251 (3 μM) (filled circle, n = 7, b), spike + DHPG (empty circle, n = 7, c), and spike + DHPG + AM251 (filled circle, n = 8, c) in DMSO-treated rat hippocampal slices. d Mean of normalized eIPSC amplitudes of the first 10 s after S-eCB mobilization protocol in b and c. DHPG or spike + DHPG (empty bar), DHPG + AM251, or spike + DHPG + AM251 (dotted bar). e Same as c, but in the presence of LY367385 (100 μM) in DMSO-treated (black circle, n = 7), AβO-treated (red circle, n = 7), and scrambled AβO-treated rat hippocampal slices (empty circle, n = 7). f The mean of the normalized eIPSC amplitudes in e. DMSO (black bar), AβO (red bar), and scrambled AβO (empty bar). g–h Same as e and f, but in the presence of MPEP (10 μM) in DMSO-treated (black, n = 7), AβO-treated (red, n = 7), and scrambled AβO-treated rat hippocampal slices (empty, n = 7). Inset: b, c, e, and g representative eIPSC traces at the indicated time points (1, 2) in each condition. Statistical tests: d Unpaired Student’s t test, *p < 0.05, ***p < 0.001; f, h one-way ANOVA with post hoc Tukey’s test, #p < 0.05, ns: p > 0.05. Data are represented as mean ± SEM

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