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Fig. 4 | Alzheimer's Research & Therapy

Fig. 4

From: Aη-α and Aη-β peptides impair LTP ex vivo within the low nanomolar range and impact neuronal activity in vivo

Fig. 4

Synthetic Aη-α and Aη-β acutely modulate neuronal activity in vivo. a The median frequency of calcium transients in the CA1 region of hippocampi of C57Bl/6 mice did not differ significantly (n.s) from baseline following superfusion (wash-in, blue) of sAη–β–CT (control peptide). Note the high degree of similarity in distribution of calcium transient frequencies as denoted by the upright histograms. The frequency of calcium transients for each individual neuron before (baseline) and after superfusion (wash-in) of sAη–β–CT is overlaid. b Superfusion of sAη–α (wash-in, red) induced a significant decrease in median calcium transient frequency and a positive skew in the distribution of calcium transient frequencies towards hypoactivity. The frequency of calcium transients for each individual neuron before (baseline) and after superfusion (wash-in) of sAη–α is overlaid. c Superfusion of sAη–β (wash-in, green) also induced a significant decrease in median calcium transient frequency and a positive skew in the distribution of calcium transient frequencies towards hypoactivity. The frequency of calcium transients for each individual neuron before (baseline) and after superfusion (wash-in) of sAη–β is overlaid. d Across all individual cells studied, the median change in calcium transient frequency following superfusion of sAη–α (red) and sAη–β (green) was not statistically different (n.s), but were significantly lower relative to the null change associated with sAη–β–CT (blue). The change in calcium transient frequency for each individual neuron after superfusion (wash-in) of each peptide is overlaid. e While the proportion of silent neurons (i.e. showing absence of calcium transients) were small during baseline conditions (grey charts), and following sAη–β–CT superfusion (wash-in, blue), superfusion of sAη–α (wash-in, red) and sAη–β (wash-in, green) was associated with a dramatic increase in the number of inactive cells. For each boxplot, the central line denotes the median with bottom and top demarcations indicating the 25th and 75th percentiles, respectively. Data for sAη–α and sAη-β-CT were previously published [3] and presented and reanalyzed in this figure for comparison purposes (denoted as). ***p< 0.001. Detailed statistics are shown in Supplementary Table S4

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